Sentence examples for manually homogenized from inspiring English sources

Xenograft tumor tissue was dissected, manually homogenized (glass homogenizer) and incubated for 30 min in protein lysis buffer (20mM Hepes (pH 7.5), 2mM EGTA, 10% glycerol, 1% Triton×100, 1mM PMSF, 200µM Na3VO4 [all Sigma], and protease inhibitors [Complete Protease Inhibitor Cocktail, Roche]) on ice.

Frozen tumors were manually homogenized with homogenization hammer and tissue powders were resuspended in 500 μl of PBS.

Tissue (1 × 2 mm) or cell line lysates were manually homogenized (PYREX homogenizer; VWR, Radnor, PA, USA) and prepared as previously described [ 32].

Prior to sampling, the soil was sieved to 2 mm (steel mesh) and manually homogenized.

The samples were then manually homogenized in extraction buffer (20 mM Tris HCl pH 8.0, 2 mM sodium EDTA, 1.2% Triton® X-100 containing 20 mg lysozyme ml−1).

Tissues were manually homogenized.

Total RNA RNAA > 200 bases) was isolated by manually homogenizing the material with micro pestles (Kisker Biotech GmbH) in TRIzol (Invitrogen).

Total RNA was extracted from tissue which had been stored in RNAlater by manually homogenizing the tissues with a mortar and pestle in TRIzol reagent (Invitrogen).

Briefly, tissue was homogenized in 200 µl of ice-cold radioimmunoprecipitation (RIPA) buffer and 2 µl of protease inhibitors (Pierce Biosciences) before being gently passed through a 23G needle and a 25G needle to manually homogenize each sample.

Five heads per genotype were manually collected and homogenized in 50 µl of a freshly prepared solution of acidified methanol (0.1% HCl).

A known weight of placental biopsy was homogenized manually in four volumes (w/v) of Tris buffered saline containing EDTA-free serine-/cysteine-protease inhibitor cocktail, diluted according to the manufacturer's instructions (Roche Biochemicals, Indiana, USA) using a homogenizer.