Ten plants were pooled for each genotype and manually ground in liquid nitrogen.
The manually ground sample can be added to the FastPrep tube with Lysing Matrix beads (see above) for additional pulverization.
In the present work, the room temperature dried algal biomass was only manually ground, which is a very mild procedure for disrupting the algae cell wall; therefore, the enzymatic hydrolysis results reported herein are quite promising.
Powdered samples were firstly mixed (1 : 100) with KBr (IR grade, Merck, Giessen, Germany) and manually ground by using an agate mortar and pestle and then uniaxially compressed (10 MPa) into pellets for examination.
After thawing, the slices were dried and then manually crumbed, grounded in a mill, and screened through 0.5 mm sieve to obtain bread powder.
MO pod shells were removed manually; kernels were grounded in a domestic blender and sieved through 600-μm stainless steel sieve.
The interactions on videos along with users' conversations while completing the task were analyzed and manually coded using the grounded coding theory [26] and each finding is described in the following sections.
Host blood was dried and grounded to powder manually.
The information gathered was manually analysed according to a grounded theory model, resulting in allocation of data into one of three domains: post-suicidal reactions, impact on daily living, and families' need for support.
An alternative to manually created ground-truth data is to generate synthetic images where the ground truth is known.
