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Ten plants were pooled for each genotype and manually ground in liquid nitrogen.
The manually ground sample can be added to the FastPrep tube with Lysing Matrix beads (see above) for additional pulverization.
In the present work, the room temperature dried algal biomass was only manually ground, which is a very mild procedure for disrupting the algae cell wall; therefore, the enzymatic hydrolysis results reported herein are quite promising.
Powdered samples were firstly mixed (1 : 100) with KBr (IR grade, Merck, Giessen, Germany) and manually ground by using an agate mortar and pestle and then uniaxially compressed (10 MPa) into pellets for examination.
Then, the dried sample was manually ground with mortar and pestle.
They were manually ground into a fine powder using a mortar and pestle on dry ice.
Similar(53)
Raw propolis (250 g) was manually grounded and placed in a flask with 600 mL of 80%% ethanol, which was placed on an agitator (Thornton, Model T14, USA) for 48 h.
Quantitative analyses are based on manually selected ground truth data.
Results with manually checked ground truths might improve slightly.
We use ten frames from each training sequence with the manually labeled ground truth points.
The algorithms are tested on real images with manually defined ground truth.
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