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Gradients were manually fractionated (0.2 mL fractions: 32 fractions/gradient) and a portion was used for absorbance determination at 260 nm.
We then manually fractionated the gradient from bottom to top into 100 μl fractions and quenched the formaldehyde in each fraction using 80 mM glycine.
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After centrifugation samples were fractionated by manually collecting 200 µl-probes starting at the top of the gradient.
The polymers were fractionated by fractional precipitation.
The gradients were fractionated from the top manually by pipetting into ∼1 ml fractions.
Active fractions were further fractionated and assayed.
CSF peptides were fractionated into 30 fractions.
Fraction S14 was further fractionated by RP-HPLC.
The alkylated protein digest was fractionated by RP-HPLC and each chromatographic peak marked in the chromatogram was manually collected and lyophilized.
Until then, the road ahead is full of booby traps and hurdles in a society that is fractionated.
Gels were fractionated into 10 parts according to molecular weight.
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