Sentence examples for manually excised from from inspiring English sources

The gel band of interest was manually excised from native PAGE gel and then digested following standard procedures.

Protein spots were manually excised from the gel, and in-gel digestion by trypsin was performed according to Wang et al. (2009a) with some modifications.

The protein fraction with the highest hexanol-degrading activity [Fig. 1f (51.4 kDa)] was manually excised from the SDS-PAGE gel and then identified by matching using reference gels/maps and peptide mass fingerprinting using a Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometer (MALDI-TOF MS, Applied Biosystems) (Klepsch et al. 2009).

Protein spots were manually excised from 2D gels.

The differentially expressed proteins spots were manually excised from CBB stained gels.

Protein spots were manually excised from Coomassie Brilliant Blue G-250 (BBI) stained gels.

Spots were then manually excised for individual in-gel digestion using trypsin [ 64].

The differentially-expressed protein spots were manually excised into 1-mm2 pieces from the silver-stained gels.

Bands from SDS-PAGE were manually excised, triturated, reduced, S-alkylated and digested over night at 37°C with modified trypsin (Sigma-Aldrich, USA).

Spots from 2-DE were manually excised, triturated, in- gel reduced, S-alkylated and digested with trypsin, as previously reported [ 88].

Silver-stained protein spots or bands were manually excised with a P2D1.5 pen (The Gel Company) from 2D and 1D gels and destained with a 1∶1 working solution of 30 mM potassium ferricyanide and 100 mM sodium thiosulfate.