Sentence examples for manually excised and from inspiring English sources

Spots showing p < 0.05 (Wilcoxon test) and not correlating with therapy13 were manually excised and destained (50% ethanol), dehydrated with acetonitrile (2 × 20 min 100 µl) and then dried at 37°C by vacuum centrifugation.

In order to identify these polypeptides each spot was manually excised and subjected to trypsin digestion.

Protein bands were manually excised and digested in-gel with trypsin using an Investigator Progest robot (Genomic Solutions, Huntingdon, UK).

After 2DGE, spots were manually excised and digested for MS/MS analysis, using methods described in the Methods S1 section.

The bands containing hemagglutinin, neuraminidase, nucleoprotein and matrix M1 protein were manually excised and reconstituted in a solution of 25 mM ammonium bicarbonate (NH4HCO3) in 50% v/v acetonitrile (ACN) at 37°C.

The spots were manually excised and processed for mass spectrometry as previously described [ 8, 17, 29].

The gel was stained with Coomassie and H3 bands were manually excised, propionylated and digested with trypsin as described before with minor modifications.

Spots were manually excised, destained and in-gel digested with trypsin [ 16].

Protein spots from the Coomassie stained 2-D gel were manually excised, destained and in-gel digested with trypsin as previously described [ 20].

Gel bands were manually excised, reduced, alkylated and digested overnight with bovine trypsin (Roche, Basel, Switzerland) as described elsewhere [20].

Protein spots of interest were manually excised from gels and digested with trypsin (Promega Inc., Madison, WI, USA).