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To revise the physical map, clone orders were manually examined using an optical map of the rice genome (Zhou et al. 2007).
All peptides identified with peptide scores of mascot >20 were manually examined using rules described previously [45].
The assembled results were manually examined using CONSED [ 14].
All sequences were manually examined using Sequencher™ 4.6 (Gene Codes Corporation, Ann Arbor, MI).
To verify positively identified residues, data were manually examined using Xcalibur software.
This analysis revealed 22 strain-specific deletions that were in the 10 strains, all of which were manually examined using IGV (Table S3).
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In such cases, promising blast hits (e-values < 1E−10) with short alignments were examined using read mapping to identify and then manually assemble fragmented gene copies.
The genes were also manually re-examined using the Btau 4.0 assembly using both RefSeq and Ensembl gene models to confirm Q-tracts and the nature of their coding sequences.
Transmembrane helices were manually examined by using available X-ray structures of prenyl converting enzymes as templates, such as prenyl synthases (cyclases), protein prenyl transferases, and the recently developed model of the all-alpha-helical E. coli UbiA prenyltransferase [16], [17].
Unique tiger salamander haplotypes were identified by manually examining alignments using SeqManPro version 8.0.2.
The 2506 beta-satellite monomers were aligned by CLUSTALW 2.0 [22] and manually examined and edited using MEGA 4.0 [62].
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