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Each reaction was manually evaluated in conjunction with the principle of metabolite availability before incorporation into the model, using 33 mitochondrial proteomic datasets from the MitoMiner database, combined with annotation from public resources and the literature.
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All of the potential episodes were manually evaluated and validated in order to exclude any false positive cases from the free text searches.
Next, we manually evaluated Online Mendelian Inheritance in Man (OMIM) entries for 314 MedGen genes not reported in the previous studies to confirm that these genes are truly associated with the autosomal recessive disorders.
The estimated counts for the novel annotations were derived by multiplying the precision rates observed in the manually evaluated sample by the total number of novel candidate annotations.
The results from the ABI3100 Prism Genetic Analyzer were manually evaluated and each sample was read in duplicate.
In addition, we manually evaluated every case of re-admission for the same diagnosis more than 2 months after the primary fracture.
Hence, among the 6,557 mapping candidates, 3,326 (50.72% of the total candidates) one-to-one mappings were manually evaluated as correct mapping candidates resulting in a precision rate of 96.46% and recall rate of 44.89%.
The sequence coverage obtained for PPP1R2P9 was 36.5% and the mascot score levels were 623.41 (in addition, spectra were manually evaluated).
In particular, computed FTs were manually evaluated by MS experts.
Then, the traveltime residual between the maximum and true peaks in the master waveform is manually evaluated.
MS/MS data of peptides which have not matched to Obp library in Mascot database search were manually evaluated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com