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Finally, the intercellular spaces were manually estimated using the following method.
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We confirmed for several selected events that a similar depth with GeoNet catalog was estimated using manually picked P and S arrivals, meanwhile a similar depth with our catalog by the envelope correlation method was estimated when only manually picked S arrivals were used.
Primers were manually designed and their characteristics were estimated using FastPCR [29].
HV was estimated using operator-defined, manually traced boundaries.
The region of interest in the field of view was manually delineated and the mean T1 value of each individual voxel was estimated using the software Siswin v.0.9 (Ringaard S, 2008).
They manually segmented the ROI only in key frames, and the ROIs in the other frames were automatically estimated using a vision technique [39].
1/S' was estimated using BlastPhen.
Confidence intervals were estimated using normal approximation.
RNA concentration was estimated using nanodrop spectrophotometer.
Divergence times were estimated using Multidivtime [52] [54].
Protein concentration was estimated using Coomassie Plus Protein Reagent.
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