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TM domain regions were deduced using TMHMM (http://www.cbs.dtu.dk/services/TMHMM) and were manually edited according to the PRINTS annotation (http://www.bioinf.manchester.ac.uk/dbbrowser/PRINTS/).
The invertebrate in silico predicted sequences were identified based on their sequence similarity for the vertebrate receptor genes using a cut-off E value higher than 10 and their sequences were manually edited according to their similarity for the homologue genes in vertebrates and EST data available (Additional file 1).
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The sites missed in the transcriptome analysis were manually inspected and all appear to be edited according to the alignment, although some at very low rates (<10%).
Sequences for each marker were compiled into contigs and manually edited using SeqManPro version 8.0.2.
▪ Ability to be human read/parsed and manually edited.
While the surface reconstructions were visually inspected, the surfaces were not manually edited, for two reasons.
Throughout the process, images were visually inspected, and any inaccuracies were manually edited.
The multiple sequence alignments were manually edited.
We then manually edited the slices.
Sequence alignments were created using MUSCLE [23] and manually edited.
All alignments were inspected by eye and manually edited.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com