Sentence examples for manually dissociated into from inspiring English sources

Blastocysts (typically 30 50 per experiment) were treated with acid Tyrode's solution to remove the zona pellucida, then incubated in 2 mg/ml collagenase IV for 20 min at 37°C, followed by Hanks'-based cell dissociation buffer (Invitrogen) for 20 min on ice, then manually dissociated into single cells using a finely pulled glass capillary.

ICM outgrowths were individually manually dissociated into small clumps and replated on new HFF feeder layers.

ICMs that attached to the feeder layer and initiated outgrowth were manually dissociated into small cell clumps with a microscalpel and replated onto new mEFs.

ICM outgrowths were manually dissociated into 4-6 smaller clumps using a microscalpel, excised from feeder cells and replated onto fresh mouse embryonic fibroblasts (mEFs).

However, different from murine, but similar to human ESCs, non-human primate ESCs proliferate at a low rate and need to be manually dissociated into small clumps for propagation.

After 10-14 days in culture, outgrowths were manually dissociated into small-cell clumps with a microscalpel and transferred to a four-well plate with a fresh feeder layer in fresh medium.

The embryos were manually dissociated in 0.9% PBS and sorted for fluorescence using a 488 nm laser with a FACSAria II (BD Biosciences, San Jose, CA).

Fixed ovaries were manually dissociated in 80% glycerol.

Lung and liver metastases were excised and manually dissociated in PBS to create an homogenous slurry.

After 2-3 days of adherent culture, neural rosettes were formed and manually isolated, dissociated into single cells with Accutase, and replated onto culture dishes coated with Geltrex (Life Technologies).

The remaining tissue was manually dissociated and incubated in 0.25% trypsin for 10 15 min.