Sentence examples for manually dissociated and from inspiring English sources

The remaining tissue was manually dissociated and incubated in 0.25% trypsin for 10 15 min.

After washing in HBSS, the cells were manually dissociated and then treated using the CMFDG kit (Invitrogen) according to manufacturer's recommendations, to yield a fluorescent product when the CMFDG substrate is cleaved by β-galactosidase.

Before transduction, hESCs were manually dissociated and incubated, in clumps, with viral particles for 2 hours at 37°C in low-attachment 24-well plates (Corning Life Sciences), with gentle rocking.

Spleens from 6 8 week-old C57BL6 mice were harvested, manually dissociated and passed through a 70-μm strainer in DMEM containing 10% fetal calf serum (FCS) (Gibco, Carlsbad, CA, USA).

In all our experiments, we removed larvae from their food by manually dissociating and floating them out of the food using a 20% sucrose solution, before washing and transferring to a new food.

Lung and liver metastases were excised and manually dissociated in PBS to create an homogenous slurry.

The method used by Krause et al. (2014) differs from ours in that it involves time-consuming isolation and purification procedures, as pieces of dermis had to be enzymatically digested, manually dissociated by pipetting, strained, and cultured to generate primary spheres.

ICMs that attached to the feeder layer and initiated outgrowth were manually dissociated into small cell clumps with a microscalpel and replated onto new mEFs.

The embryos were manually dissociated in 0.9% PBS and sorted for fluorescence using a 488 nm laser with a FACSAria II (BD Biosciences, San Jose, CA).

Manually dissociated ovaries were mounted on slides and analyzed as above using the same microscope.

However, different from murine, but similar to human ESCs, non-human primate ESCs proliferate at a low rate and need to be manually dissociated into small clumps for propagation.