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For library construction, all nectaries were manually dissected from B. rapa flowers (an example dissection is available in Video S1), and processed as described in Materials and Methods.
The maternal basal plate (decidua) was manually dissected from the fetal placental tissue.
Median and lateral nectaries were manually dissected from open B. rapa flowers (equivalent of Stage 14 15 in Arabidopsis [27]) and pooled in separate tubes containing RNAlater™ solution (Ambion, Austin, TX) on ice, and stored at −20°C prior to RNA extraction.
Normal thyroid tissue and PTC area were manually dissected from 3 serial sections.
In addition, ICM and TE were manually dissected from day 9 blastocysts.
Melanoma tissue was then manually dissected from surrounding normal tissue with a sterile blade.
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Formalin-fixed paraffin-embedded (FFPE) thyroid tissue samples were manually dissected for DNA isolation.
To maintain a useful supply of pluripotent stem cells, the undifferentiated colonies, or portions of those colonies, must be manually dissected away from those that have begun to differentiate, then re-plated for further growth.
The remaining periostracum and proteinaceous components of the shell were manually dissected away from the animal with fine dissecting forceps.
DNA was isolated from manually dissected tumour areas from 5 μm sections of FFPE tissues with a tumour cell content of at least 70%.
Here we have manually dissected individual ICMs from TE.
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