For patch clamp electrophysiology on L1 (22 24 h AEL) larvae, animals were manually dissected by gluing (Histoacryl Blue, Braun Germany) them to sylgard-coated coverslips under standard Drosophila saline (135 mM NaCl, 5 mM KCl, 4 mM MgCl2, 1.8 mM CaCl2, 5 mM TES, 72 mM sucrose).
Therefore, animal cap explants of blastula embryos were manually dissected and dissociated by trypsin treatment.
Pre-amplification was initiated by 10 min hot start at 95°C, followed by 14 (manually dissected) or 17 cycles (LCM) of denaturing for 15 s at 95°C and annealing/extending for 4 min at 60°C.
To validate the bioinformatics tools, we randomly sampled a subset of the total data and manually dissected the raw reads one by one to identify the CDR3 sequences.
Xenopus laevis oocytes were obtained by surgery, manually dissected, and defolliculated with collagenase (Sigma, St . Louis MO).
For 19 patients, we obtained samples from tumour and healthy tissue blocks, the tumour areas were selected on the haematoxylin/eosin sections by microscope and manually dissected.
Visualization of four rounds of nuclear division during T. reesei ascospore formation/maturation. (A-G) Developing asci were manually dissected, stained with DAPI, and then visualized by fluorescent microscopy.
For library construction, all nectaries were manually dissected from B. rapa flowers (an example dissection is available in Video S1), and processed as described in Materials and Methods.
After identification of at least 75% tumor area by a pathologist, tumor tissue was manually dissected from five consecutive 10 μm sections of the paraffin-embedded tissue.
Similarity of hESC-derived cardiomyocytes to adult cardiomyocytes was also described by Synnergren et al. [27], who analyzed manually dissected beating areas that are enriched for cardiomyocytes but also would be expected to include a large percentage of non-cardiomyocytes.
Following microwave irradiation, the medial prefrontal cortex (MPFC) was manually dissected (60 90 mg) and homogenized with 0.1 M HCl/methanol (2 1 vol/wt) followed by extraction with ethanol.
