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This is particularly important for the TE and ICM samples since these were manually dissected and confirms the reproducibility of the dissection.
The pancreas was then manually dissected and washed multiple times in PBS.
Within a week, neural precursor cells were manually dissected and grown in suspension, forming neurospheres.
Adipose fat pads (perigonadal, perirenal, retroperitoneal, mesenteric and mediastinic [82]) were manually dissected and weighted.
The rosettes were manually dissected and expanded in NSC proliferation medium on defined substrate (Fig. 1E).
For immunohistochemistry and confocal microscopy, animals were manually dissected and fixed in Bouin's fixative as previously described [46], [47].
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Pearson's correlation coefficient was used to determine the temporal correlation of 1) expression of porcine and bacterial genes, 2) of porcine gene expression measured in manually dissected samples and LCM samples, and 3) of mRNA and protein levels of cytokines.
Expression of TLR4, IL1B, IL6, IL8, and SAA in manually dissected material and LCM material was highly correlated (Pearson's r > 0.72, P < 0.05); CD14 and TNF expression was also positively correlated in the two sample types, although not significantly.
Grains from 0, 4, 8, 10, 12, 14 DAF were manually dissected into maternal and filial parts and whole caryopses were sampled at 16, 20 and 24 DAF.
For the latter, the different plant parts are typically manually dissected, dried, chopped and ground [ 8- 11].
They were manually dissected in PBS and fixed in a 1% glutaraldehyde in PBS buffer for 20 min, rinsed in PBS, and mounted in a NaCl/glycerol solution.
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