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In this example, since PTEN is manually designated as a leaf node unlike the other three input proteins designated as main nodes, the only interactions presented for PTEN are those with the main nodes.
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Efforts are already underway that manually designate some genomes as Reference Genomes, such as Gene Ontology Reference Genomes [4] and Quest for Orthologs [5].
Homologous sequences were manually extracted, translated and designated as either class I or class II pilE based upon relatedness to known pilin genes and the presence of the SM1 motif (EYYLN) in the translated sequence.
Using the graphical view within the SDS software (ABI), each group of alleles could be manually selected and each sample designated as homozygous wild-type, heterozygous or homozygous variant.
Regions of interest (ROI) were manually selected over the perinuclear region, designated as perinuclear (PN), or plasma membrane region, designated as membranous (M).
Each of the new entries is examined manually and either added to the database or designated as false positive.
Many are designated as historic districts.
They are often designated as wild cards.
It was designated as C8.
One rat was designated as the "encoder".
Other similar channels were designated as duplicates.
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