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The MR images were preprocessed, including a down sampling to 1 mm cubic voxels [4], and the whole cranial vault manually delineated in all slices following the dura mater in sagittal orientation.
For each patient, VOIs were manually delineated in the LV (VOILV) on the SPECT images for RBM dosimetry and for one tumor lesion (VOItumor) on the co-registered CT images for the tumor dosimetry.
Tumor and heart were manually delineated in 3D reconstructed images of each mouse, and time-activity curves (TACs) were derived from the regions of interest (ROI) using ASIPro VM™ software (Concorde Microsystems).
Lungs profile was manually delineated in each cross-sectional lung image in order to identify the regions of interest.
SC was manually delineated in each image allowing a purpose-designed software code to calculate the direction normal to the skins surface at each point.
Trails were manually delineated in each image at a 1 3,000 resolution and compared to ground observations made in September 2009.
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The tumor area is delineated in yellow.
For comparison, the same three tumours in each patient were manually delineated by an oncologist in CT or MR images originally acquired for treatment follow-up so as to estimate a morphological volume of the tumour.
Quantitation of positive areas was done by using the Image J software in manually delineated blood vessels that have a diameter less than 10 μm.
The segmented grey matter was also used in ROI extraction to only include grey matter voxels in the manually delineated cortical ROIs described above.
The manually delineated intracranial areas are highlighted in blue and pink.
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