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The fibre tips or cytoplasm regions were manually delimited on sum projections of the pixel intensities over z-stacks.
In this manner the renal area was manually delimited on its external surface in the six sequential planes, and the equipment provided the image reconstructed three-dimensionally as well as its volume in cm.
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Motoneurons were manually delimited and mean grey intensity was measured on the human SOD1 channel with the ImageJ software (National Institutes of Health, USA).
For each Atypical OBP, the boundary between the N-term and C-term PGP/GOBP domains was manually delimited.
Mean human SOD1 fluorescence intensity measured within manually delimited ChAT- and RFP-positive motoneurons revealed significant reduction in human SOD1 expression in treated mice (Fig. 3B and D).
The granuloma area was manually delimited, captured by a CCD camera using bright field microscopy, and automatically processed with IMAGE-PROPLUS.
These two copies are harboured by identical 40 kb DNA fragments delimited, on one hand, by an IS element.
The surrounding area is delimited on the south by Syria and on the east by the Euphrates River.
The sample areas were delimited on aerial photographs, and some were checked by GPS during the field survey.
In each of the images, the calibration pattern region has to be delimited manually by drawing a polygon that encloses the area.
The region was delimited manually and expressed as TV.
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