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Firstly, we manually defined the six points of a standard face.
We extracted no more than 20 most-similar sequences for each consensus sequence, aligned them, and manually defined the Helitron element boundaries (Supplementary Fig. S2).
A method proposed by Shionyu-Mitsuyama et al. (2003) and its extension by Saito et al. (2006) manually defined the fragments for carbohydrates and nucleotide bases, respectively, but these fragments, such as glucose, galactose, guanine, adenine and others, only correspond to a few specific ligands.
To generate masks for each MB compartment, we averaged the registered intensities of MB extrinsic neurons projecting to the same compartment and samples representing the same KC cell type; we manually defined the borders between neighboring compartments after applying a Gaussian blur filter in 3D (sigma = 2).
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Each of the two types of VOIs was generated by manually defining the sphere centre from the images and then producing a spherical VOI mask based on the sphere radius.
Once the raw data are in an acceptable video file format, we select a reference frame and manually define the rotation, if any, the crop indices (crop area), and the background for subtraction.
As a result, individual SUVmean during infection could be underestimated by inclusion of voxels with lower metabolic activity from surrounding tissue when manually defining the LN boundaries on CT images (Figure 6e).
Each patient's T1-weighted MR image was co-registered to their 18 F-FDG PET using VINCI software (Max Planck Institute, Cologne, Germany) and subsequently used to manually define the regions of interest (ROIs) of the pons, occipital lobe, and cerebellum in normal subjects (Figure 1).
We manually define the cytotoxic conditions and select the genes using the computational model.
While data sets are available for Affymetrix chip for some species, for other platforms, such as the custom cDNA microarrays, one would need to manually define the gene sets, which can delay procurement of downstream information or introduce errors.
17 20–24 However, with this software the user must manually define the gland region for each patient.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com