The strip was then manually cut in 58 (PR1) and 64 (PR2) fractions with a scalpel, and gel pieces were placed in polypropylene tubes containing 70 μl 0.1% TFA.
The 68 differently expressed spots were manually cut from gels and characterized by LC ESI MS/MS.
Furthermore the upright microscope is used to visualize manually cut sections of tissue, and the processing and sectioning of samples can result in a loss of tissue integrity.
The somata and any obvious artifacts were manually cut from each image, and then we obtained a count of the black pixels.
As in Figure 6, individual cells representing the staining patterns observed in each image were manually cut from the images, and labeled based on their apparent organelle (o), membrane (m) or nuclear (n) staining patterns.
To assemble figures, individual cells representing the staining patterns observed in each image were manually cut from the images, and labeled based on their apparent organelle (o), membrane (m) or nuclear (n) staining patterns.
The hearts were placed into a petri dish containing the digestion buffer and manually cut into small pieces.
Fresh uncured ham was purchased from a local supermarket at Wuxi, Jiangsu, China, and manually cut into 7 × 8 cm pieces.
The fresh purchased ham was sliced in a Hobart 2712 12′′ semiautomatic slicer (Hobart Mfg. Co., Troy, OH) and manually cut into pieces of 7 cm × 8 cm per side with total surface area of 112 cm.
Samples were manually cut with a razor blade, and sections were mounted with conductive graphite adhesive (Leit-C after Göcke, Plano, Wetzlar, Germany) on an aluminium specimen stub.
