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Outer surface of the assembled channel has been manually covered with a positive-tone photoresist (AZ P4620) and the whole structure was dipped in aluminum etchant (Aluminum etchant type A, Transene) at 40°C.
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Embryos were manually dechorionated, covered with mineral oil on a glass slide, and flattened with a cover slip.
The vitteline membrane and all debris were manually removed and covered with a cover slip.
Images were inspected manually using GenePix Pro 6.0 software (Axon Instruments), and any spots that were covered with hybridization artefacts were removed and were not included in the further analysis.
Research by Human Rights Watch in Zamfara in late 2011 found that children are exposed to lead when they process ore in the mines, when their miner relatives return home covered with lead dust, and when the lead-filled ore is manually or mechanically crushed at home.
Individual cerebral ganglion F-cluster top layer neurons(48) were manually isolated and placed onto an AnchorChip sample plate at a region that was not covered with MALDI matrix.
Images were inspected manually using GenePix Pro 6.0 software (Axon Instruments, Union City, CA), and any spots that were covered with hybridization artifacts were removed and not included in further analysis.
Protein spots from Coomassie stained preparative 2-D gels were selected and excised manually using a clean scalpel or razor blade (Fig. 1G), put into 1.5 ml Eppendorf tubes, covered with water and store in the freezer until digestion.
Staining was visualised using the chromogen 3,3′-diaminobenzidine for 10 min, counterstained with haematoxylin (EnVision FLEX; Dako) for 5 min and manually cover-slipped (Surgipath) with DePeX mounting medium (VWR International, Poole, UK).
Cover with remaining dough.
Cover with them water.
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CEO of Professional Science Editing for Scientists @ prosciediting.com