Sentence examples for manually coupled from inspiring English sources

For all peptide conjugates, 5-FAM was manually coupled at the peptide N- or C-terminus (after Mtt removal) with 5-FAM/HBTU/DIEA at a ratio of 4 4 10 relative to the peptide, shaken overnight at room temperature.

22,26,36 Here, the support 8 was manually coupled with the corresponding Fmoc-protected amino acid using the tetramethyl- O-(1 H-benzotriazol-1-yl)uronium (HBTU) reagent.

In this study, 5′-dimethoxytrityl[N3]-2′-deoxycytidine-3′-[ 2-cyanoethyl - N, N-diisopropyl)]phosphoramidite was manually coupled to an Ac-dC-CPG solid support, followed by standard deprotection techniques performed on the DNA synthesizer (Scheme S2).

The selectively S-protected A- and B-chains were of high purity as determined by analytical RP-HPLC and MALDI-TOF MS. An Fmoc-propargylglycine was manually coupled onto the N-terminal end of the B-chain prior to cleavage from the solid support and subsequent crude peptide purification.

After completion of the BBN fragment, the Fmoc-NH- PEG 1-CO2H and Fmoc-NH- PEG 1-CO2Hwere Fmoc-NH- PEG 1-CO2H the peptide on resin.

The FITC label was coupled manually using an on-resin approach.

For the stapled peptide, Fmoc-S5-OH was coupled manually in threefold molar excess in the presence of 2- 1H-benzotriazole-1-yl -1,1,3,3-tetramethyluronium hexafluorophosphate, 1-hydroxybenzotriazole and DIEA.

Ac-IK PEG3-Cys- β-Ac-IK PEG3-CysAc-IK PEG3-CyseArg-Y-NH2/Ac-PYY(PEG3-Cys- β-Ala-Biotin -Pqa-RHYLNWVTRQ-MeArg-Y-NH2/Ac-PYY PEG3-Cys)H(Trt)Y(tBu)LN(Trt)W(Boc)VT-(But)R(Pbf)Q(Trt)-MeArg(Mtr)-Y(tBu)-TentaGel S RAM resin, the peptide was coupled manually 2 times 30 min with 66.5 mg (3 equivalents [equiv.]) Fmoc-12-amino-4,7,10-trioxadodecanoic acid (PEG3-spacer), 57.0 mg (3 equiv).

Manual curation will continue to provide high-quality UniProtKB data, ensuring that users have access to accurate and consistently annotated experimental information coupled with manually verified sequence analysis predictions.

Two lasers are manually focused using a camera coupled with the device in order to set the analysis region.

Briefly, high-pressure experiments were conducted with a 3 ml capacity model MS-1 stainless steel reaction vessel coupled to a manually operated model HiP high pressure hydrostatic generator (High Pressure Equipment Company, Erie, PA, USA).