Sentence examples for manually counted using from inspiring English sources

CA, USA) and then reacted at 4°C for 20 min on STR-functionalized substrates (SiNW, QNP, and planar glass) prior to loading the A549 cells, which were manually counted using a hemocytometer with approximately 10% error (Hausser Scientific Co., PA, USA), into the PDMS cell-capture chambers (Figure1c).

OMP and β-tubulin immunopositive cells were manually counted using ImageJ software in 200 µm×200 µm region.

For all ages, the number of motifs that each bird sang throughout the entire day on days 2 5 was manually counted using Audacity (v1.3).

Micrographs from microtiter plate wells (9 fields per well) were obtained using confocal apotome microscopy (Zeiss) and trophozoites were manually counted using ImageJ software's plug-in "cell counter" (http://rsb.info.nih.gov/ij).

Slices were rinsed in PB, mounted onto microscope slides, embedded in mounting medium (Prolong Gold AntiFade, Invitrogen, Carlsbad, CA), and coverslipped before AVP- and VIP-immunoreactive (-ir) cells were manually counted using an Olympus confocal laser scanning microscope (FLUOVIEW FV300).

The colonies were manually counted using a microscope.

For blood vessel length, images were light corrected (Image J, version 1.43u) then analyzed for length using angiogenesis tube formation (Metamorph Software, version 7.7.0.0, Molecular Devices, Inc .. Branch points were manually identified and counted using Image J (cell counter).

TUNEL-positive nuclei were manually counted, and the total nuclei were counted using ImageJ software (NIH).

The number of cracks in the gage length (80 mm) were manually counted by using a microscope on both sides of each specimen.

The number of cells expressing receptors and the total number of cells in the sensory epithelium were manually counted and used to calculate the percentage of expressing neurons in supplementary figure S5, Supplementary Material online.

To calculate cellular density, we manually counted cone photoreceptors using an image-processing program (ImageJ, National Institutes of Health, Bethesda, MD, USA).