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The data were first analysed using the automated LinRegPCR functions and subsequently manually corrected where required (as suggested by the LinRegPCR user manual, version 12.x).
was used to identify assembly errors, which were manually corrected where possible.
The resulting labels where manually corrected where necessary, using both the T1 and the T2 weighted scans, to improve the results based on local alignment.
Masks were inspected and manually corrected where necessary.
Multiple alignments were obtained with ClustalW [ 55] and manually corrected where necessary.
Multiple alignments were visually inspected and manually corrected where necessary by means of the BioEdit package [ 27].
Similar(51)
This annotation was manually checked and corrected where necessary, which took about 5 person-hours.
Genes with flagged features (proteins ≤50 aa, internal exons ≤6 nt, introns ≤20 nt, introns ≥1500 nt, exons spanning gaps in the assembly, internal stop codons, overlapping other coding sequences, overlapping ncRNAs (tRNAs, rRNA, or other)) were manually reviewed and corrected where supported by the evidence.
Each annotation and contig assembly was manually corrected, when necessary.
Alignments were manually corrected when necessary with GeneDoc 2.6 [ 40].
The identified database records with errors were manually checked against the data capture forms and corrected where needed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com