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Then, missing values were manually corrected using small value correction only if all the values were missing in the biological replicate.
Sequence alignments were generated using clustalW and manually corrected using BioEdit.
The 50 DNA sequences of each gene partition (rbcL and rps4 genes, trnL intron, trnL-trnF intergenic spacer) were aligned separately, visually inspected and manually corrected using the Mega 4.0 software [51].
However, these scorings were easily manually corrected using the GenomeStudio software.
Sequences were aligned using MultAlin and manually corrected using GeneDoc v2.7.0 (Nicholas et al. 1997).
If the VOIs included physiological FDG uptake, then they were manually corrected using the 'interactive' tool.
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Spectra were Fourier transformed with 0.3 Hz line broadening, automatically phased and manually baseline corrected using the TOPSPIN software.
These alignments were manually inspected and corrected using GeneDoc version 2.6.002 (Nicholas 1997).
DNA electropherograms were manually inspected and corrected using Chromas program (McCarthy, Queensland, Australia).
All plasma H NMR spectra were manually phased and baseline corrected using VNMR 6.1C software (Varian , Inc.
Spectra were processed using MestReNova version 9.0.1 (Mestrelab Research S.L., Santiago de Compostela, Spain) and were manually phased and baseline corrected using a Whittaker Smoother base point detection with spline fitting.
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