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When manually constructed by one or more annotators, it receives the denomination of a gold-standard corpus.
Within each of these 35 datasets, diploid genotypes were manually constructed by randomly pairing two haploid DNA sequences (i.e., alleles), to generate a diploid genotype.
Their gene structures were then manually constructed by using both annotation and the gene structures of H. sapiens and A. thaliana as references.
For each clade (usually, species) forming the group to be tested, trees with alternative topologies were manually constructed by joining the clade in question to every other major group in the tree.
We analyze the results in terms (1) of the minimum evolution criterion, (2) how close the genome halving result is to the final (local) minimum and (3) how close the final result is to an ancestor manually constructed by an expert with access to additional information.
All such developments rely on accurate definition of antigen-specific T-lymphocyte responses, and the HLA libraries for Asian variants reported here are essential for the characterization and enumeration of clinically relevant antigen-specific CD8+ T cells 8. Conditional ligands were manually constructed by standard Fmoc-based solid-phase peptide synthesis.
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Their gene structures were then manually constructed both by using annotation (if available) and by aligning their sequences with those of other species.
Some of these reactions were missing because they represent either processes specific to C. thermocellum, or processes that must be represented by manually constructed, lumped reactions.
However, the alignment from which we inferred the gag tree was manually constructed and its accuracy tested by comparative analyses.
Gene diagrams of regions between fkbp and lpxC and katA and prlC by were manually constructed and drawn to scale using Microsoft PowerPoint.
Cohen et al. (2009) selected triggers by iteratively testing manually constructed patterns.
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