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Four nrd-like genes were found in ϕpp2: one was found explicitly by the RAST and three others were implicit but manually confirmed with PSI-BLAST searches.
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Variable domain sequences were translated into peptide sequences using a self-written Perl script, and manually confirmed by comparing with the literature [ 4].
Confident detection of MPM candidate biomarker peptides in serum was manually confirmed based on transition co-elution with simultaneously monitored heavy isotope-labeled synthetic peptides with matching sequences (SpikeTides_L™, JPT Peptide Technologies) spiked in the samples before MS analysis.
In a few cases, e.g., for some molecular features with high fold change and high significance, integration and alignment were manually confirmed and were in good agreement with XCMS results.
To check the accuracy, we manually confirmed whether these genes are associated with the essential hypertension.
Identifications in consensus with both Mascot and MassMatrix were manually confirmed by spectra annotation.
Residue assignments were generated manually and confirmed with the PINE server v1.0.
We manually confirmed all the trees.
All the identified phosphorylations have been manually confirmed.
All genotyping results were manually confirmed by at least two researchers independently.
All genotyping results were manually confirmed by at least two independent researchers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com