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Based on the quantified p53 fluorescent trajectories, we classified the dynamic mode of nuclear p53 manually by eye.
All alignments were then improved manually by eye in BioEdit before the outgroup sequences were added and aligned.
Sequence data were aligned and edited manually by eye and using clustalW implemented in BioEdit v7.0.53 for Windows [ 51].
All alignments were done with Sequencher v4.7 [ 86] and Bioedit v7.0.9 [ 87, 88] and checked manually by eye.
The motor RSN was identified manually by eye and then confirmed by using spatial cross-correlations against a previously defined motor map (Beckmann et al., 2005).
Raw sequences were checked manually by eye using BioEdit [ 36] and assembled using the SeqMan program from the laser gene package DNAStar (Madison, USA).
Similar(50)
All sequences were aligned using Clustal X [50], then manually adjusted by eye.
Sequences were edited in Sequencher (version 4.8; Gene Codes), and any polymorphisms were manually checked by eye.
Validation and modification of the results were manually performed by eye.
Sequences were aligned using MUSCLE with default settings [ 41] and manually adjusted by eye.
Sequences were edited and aligned using BioEdit v7.5.0.2 [ 89], and manually checked by eye.
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