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Dule et al. [22] manually assigned the ROIs on vehicle hoods from the detected foreground objects.
Activation of the epoxide ring oxygen by protonated D101 is a prerequisite for ring opening in LEH; therefore D101 was manually assigned the protonated (neutral) form.
For each FRAP time series, we manually assigned the bleached region as a region of interest (ROI) and calculated the mean intensity of the ROI.
We manually assigned the GO categories to these five groups and determined whether these groups were over-represented among the lineage specific genes versus the non-lineage specific genes using a False Discovery Rate of 1% (See Additional files 6, 7 and 8).
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The dynamic classification provides automation and eliminates the need to manually assign the jobs thereby reducing the delay and, subsequently, the cost associated with manual resource creation and release.
Although we can manually assign the bond types for the co-crystalized ligands, the procedure is tedious and time-consuming if one wants to deal with a large number of structures.
'Unknown' annotations are created by manually assigning the root term of a GO aspect, which are the broadest terms that exist: 'molecular_function' (GO 0003674), 'biological_process' (GO 0008150), and 'cellular_component' (GO 0005575).
To distinguish the cartilage from the bone, the selected slices were segmented by manually assigning the lower and upper thresholds to isolate the voxels within the cartilage peak, which unavoidably contained a few bone marrow voxels.
The contigs, information regarding each contig, the BLAST and SignalP results were combined in a hyperlinked Excel spreadsheet and each contig annotated by manually assigning the most likely predicted function based on BLAST results.
The fragment ions were manually assigned to the mass spectra with the help of the GlycoWorkbench fragmentation tool.
Where components were not disjoint (i.e. were connected to multiple modules), the component was manually assigned to the module with which the majority of nodes were connected.
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Justyna Jupowicz-Kozak
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