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PGB02 was manually assembled into 2 contigs.
Contigs were then manually assembled into the complete circular genome sequence.
Then the two large contigs were manually assembled into the complete circular genome sequence.
Using the graphical multiple sequence editor SeaView [ 31], the padded reads were manually assembled into the defining gene sequences.
Finally, 11 BAC clones with minimized overlap were chosen and manually assembled into a contig with minimal tiling path.
Sequences from the same clone were manually assembled into contigs with the help of Seqtools http://www.seqtools.dk/.
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We then manually assembled the obtained sequence reads into a contig sequence.
The delineated pathways were then combined into plausible signalling cascades and initial sub-models were manually assembled using the PathVisio software.
These 15 contigs were refined manually and assembled into seven larger contigs.
Ambiguous nucleotides (nt) of the L-P1 sequences were resolved manually and assembled into a contig using the SEQUENCHERTM 4.7 DNA sequence analysis software (Gene Codes Corporation, Ann Arbor, MI, USA).
Nonetheless, this might not be the case in manually assembled camera rig studied in this paper.
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