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Whole mount RNA in situ hybridization was performed manually as described [41].
The plates were then stained with crystal violet solution (Sigma Aldrich, St . Louis MO) and colonies were scored manually as described previously [35].
Spots of interest were excised manually as described previously [28] and subjected to mass spectrometric protein identification Proteins were identified by two approaches as described recently [29], [30].
All encoded globin sequences were aligned manually as described previously [ 42, 43].
Lesions were defined manually as described in Geva, Baron, Jones, Price, and Warburton (2012).
For IHC staining, TMA slides were processed and stained manually as described earlier [ 36].
Similar(38)
Estimation of the number of GFP photobleaching steps was performed manually as describe above, with the same criteria used for exclusion of a subset of traces from the analysis.
Genotypes were automatically called with the SpectroCALLER software (Sequenom), and manually checked as described in Silander et al [32].
The basis of our analysis was the disease datasets, comprising of unique interactors of disease-gene products, which were manually verified as described before.
Finally, for the LK genes without cDNA and ESTs matches, the predictions were manually performed as described by Meyer et al. [30].
Movement responses were manually scored as described above.
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