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Samples were manually arrayed into three 96-well master plates.
The BAC library consisted of a total of 49,152 clones, which were manually arrayed into 128 384-well plates.
At 20 h post-fertilization (hpf) (which coincides with the onset of the primordium migration), cldnb EGFP embryos were manually arrayed into 96-well dishes (two embryos per well) using a 200-µl wide-bore pipette tip and treated with test compounds at a final concentration of 10 μM.
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Manually isolated colonies were randomly picked and arrayed into 96-well microtiter plates containing liquid selective medium.
Next, after manual removal of the chorions, cldnb EGFP embryos were arrayed manually into the 96-well plates (two embryos per well) in 200 μl of system water at 20 hpf, resulting in a final screening concentration of 10 µM.
According to tissue types and experimental conditions, the 2364 array data were manually classified into 133 experimental series, whose complete listing is available as Supplementary Table S1.
The data consists of 5,372 samples profiled on HG-U133A array platforms; As described in [ 80], the data were normalized and manually labeled into 369 biological groups covering a wide range of healthy/cancer tissues, conditions, and cell lines.
That array of sensors also means that users didn't have to manually switch into a discrete sleeping mode, which has honestly always been a pet peeve of mine — I'd love to gain some deeper insight into what few hours of sleep I manage to get, but I tend to pass out before flipping the sleep switch.
It is manually woven into the towel.
Phone numbers can be manually entered into the Jitterbug or the company can do it for consumers.
Proteins were manually grouped into 25 metabolic pathway categories.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com