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Our study also provides evidence that dN/ dS ratios calculated from automated gene annotation are similar to those calculated from manually annotated genes.
Of these, manually annotated genes on the HGS were followed by ".nc", and others were followed by ".nd".nd
We manually annotated genes with homologs in other fungal genomes that have known relevance to fungal virulence and life history.
To compare signatures of selection and expression between different categories of immune genes, our manually annotated genes were classified according to their specific function.
The GENCODE consortium in the framework of ENCODE (encyclopedia of DNA elements) project estimated, already in 2012, the human catalog of lncRNAs comprising 9277 manually annotated genes and producing 14880 transcripts [ 4].
Therefore, we also compare the results obtained from the analysis of manually annotated genes with data from the same genes generated by automated gene predictions available in the Ensembl database.
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GENCODE has a higher proportion of manually annotated gene models than RefSeq and includes more novel splicing features.
Each manually annotated gene prediction in the OGS underwent basic validation using CodonW (http://codonw.sourceforge.net/).net/
The FlyBase annotated gene set consists entirely of manually annotated gene models, with the exception of some classes of small non-coding RNAs.
We do not use gene predictions constructed by assembling a cluster of reads into a full-length transcript as support for building manually annotated gene models.
CTD-2369P2.8 is a manually annotated gene from the Havana (the Human and Vertebrate Analysis and Annotation) project, and it is longer than ICAM4.
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