The pancreas was then manually dissected and washed multiple times in PBS.
Cells collected in British Columbia were manually isolated and washed three times in f/2-medium.
The amnions were cut into small pieces (2∗2 cm) and then manually separated and washed extensively in phosphate-buffered saline containing 100 U/mL penicillin and 100 μg/mL streptomycin (Beyotime, China).
After laser ablation, individual apical or basal cells were collected manually and immediately washed to avoid possible contamination from the broken cells.
Flowers were separated manually from aerial parts and washed with tap water prior to freeze drying.
Briefly, spots from 2-DE were manually excised from gels, minced and washed with water.
Coomassie blue stained protein spots were manually excised from the gels and washed in H2O/MeOH/acetic acid (47.5/47.5/5) until destaining.
Protein spots were excised manually from 2D-GE gels and washed with 100 mM NH4HCO3 for 15 min.
In brief, bands of interest were manually excised from the gel and washed with deionized water followed by two washes with 100% acetonitrile for 15 and 2 minutes, respectively.
Spots which increased or decreased in volume (protein content) by two-fold or more were manually excised from the gels and washed four times with 50 mM NH4HCO3 containing 50% v/v acetonitrile (ACN) to remove SDS and dye.
