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The computed results were reviewed manually and validated.
Phylogenetic trees were constructed manually and validated by the Network 4.500 program software.
Some MeSH to VCM alignments were performed automatically but others were performed manually and validated.
Homoeologous specific primers were designed manually and validated with Oligo Analyzer (Gene Link) to avoid secondary structure formation.
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The site mapping spectrum was manually inspected and validated.
The mouse reconstruction was then manually completed and validated.
These cell-specific networks were manually curated and validated based on known cellular metabolic functions.
All completed questionnaires were entered into a database immediately after being manually coded and validated.
MS/MS data were manually curated and validated using the Scaffold programme (Proteome Software).
The initial 124 disease list was manually collected and validated against the Medical Subject Headings (MeSH) database [ 24] in order to determine its neurological and psychiatric relationship.
In total, 137 manually annotated and validated Physcomitrella patens gene structures are in the cosmoss.org genome browser track "Ppref genes".
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