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Proteins of interest were excissed manually and processed as described [16].
Fifty heads and ovaries were separated manually and processed for total RNA extraction and reverse transcription as described above.
Classes showing well-stained top, right, or ring views were selected manually and processed separately in subsequent steps.
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And even if the customer pays the merchant directly using M-Pesa, the payments take place outside of the store's usual systems and need to be manually recorded and processed.
The spots were manually excised and processed for mass spectrometry as previously described [ 8, 17, 29].
Coomassie stained bands were manually excised and processed for mass spectrometry as follows.
Protein spots from 2D SDS-PAGE were manually cut and processed using In-Gel tryptic digestion kit (Pierce).
In addition to the currently published repositories such as BLAD (14) and CARD (13), some previously reported β-lactamase databases contained manually curated and processed β-lactamase data.
One repeat is performed per parallel job, and any output files generated must be manually collated and processed by the user.
PCR products were analyzed by gel electrophoresis: aberrant bands were manually excised and processed using the QIAquick Gel Extraction kit (Qiagen) for sequencing.
Multiple sequence alignments (MSA) were manually edited and processed by Gblocks at http://molevol.cmima.csic.es/castresana/Gblocks_server.html (last accessed April 28 , 2015 (Talavera and Castresana 2007) to remove poorly aligned and divergent regions, except for PA28 for which BMGE (block mapping and gathering with entropy; Criscuolo and Gribaldo 2010) with a 0.6 cut-off value was used instead.
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