Then, PGCs were collected manually with a micropipette and observed under an MZ16F fluorescence stereomicroscope (Leica) equipped with a DS-5Mc digital camera (Nikon).
After we found fewer changes in conformation of ligands at the docking sites, we began manually analyzing the hydrogen bonds and observed weak C-H.O interaction with an H.O distance of >1.85 Å.
The fidelity of our rules was reinforced by the comparison of simulated and observed time series data, first manually, then automatically by the attribute exploration algorithm.
The principal vibrissa was determined by manually deflecting individual whiskers and observing resultant multi-unit activity.
Each sequence was generated twice and independently from regional cancer registry data (automatically as "algorithm sequences") and from medical record (manually as "observed sequences").
However, the current patch clamp method required a skilled technician to manually manipulate the pipette, apply the appropriate pressure, and observe the electrical resistance [8].
Pairs were manually observed for 30 min, and copulations were recorded every minute.
Gal-file grids were manually adjusted observing both colors and Cy3/Cy5 ratios were normalized to human and murine IgG as non-specific controls.
While especially for high cell counts, the manual counting showed a deviation of up to 400 cells/mm2 (41% variation), algorithm-determined T cell numbers generally lay in between the manually observed cell numbers but with perfect reproducibility.
There are, however, regions inside the head not covered by these ROIs, which must be manually observed during the analysis.
