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The first spreading machines in the late 1890s, often built of wood, carried fabrics in either bolt or book-fold form as the workers propelled the spreading machines manually and aligned the superposed plies vertically on the cutting table, thus making the cutting lay.
Sequences were assembled manually and aligned using NCBI Blast and Vector NTI.
The results of Sanger sequencing were removed vector sequences manually and aligned using Blastn and MegAlign in Lasergene packages.
We assembled sequences with Seqman (DNASTAR), inspected all polymorphisms manually and aligned sequences with ClustalW (in BioEdit [ 38]), with adjustments by eye.
All sequences were cured manually and aligned to L. maculans genome sequence using BLASTn with a cutoff e-value of 1e−10.
Sequences were assembled manually and aligned against the complete mt genome sequence of A. suum (GenBank™ accession number NC001327) using the program Clustal X to identify gene boundaries.
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(1) New sequences were manually added and aligned to the previously published [ 4] multiple alignement using the BioEdit sequence alignment editor [ 31].
Sequences were aligned manually and also aligned using Clustal W [ 92] with default parameters.
Newly obtained EEF2 and HSP70 sequences were manually assembled and aligned to sequences downloaded from GenBank using MacClade ver. 4.08 [97].
Sequences were then manually adjusted and aligned to the Greengenes 16S rRNA gene database [66] in ARB [67].
After pre-processing, the data were manually assembled and aligned using CODONCODE CodonCode Corporationn, Dedham, MA).
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