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All the sequences were then manually aligned by BLAST search against the assembled contigs to predict potential genes involved in the taxol biosynthesis.
Homologous sequences were manually aligned by Sequencher v.4.1.
Nucleotide sequences were translated into amino acid sequences and manually aligned by using GeneDoc software (20 ).
Once registration was complete, the separate layers were manually aligned, by using a minimum of three reference points.
In the first method, samples from different experiments in the same species were manually aligned by overlaying growth curves for each experiment.
The sequences were aligned with previously published sequences from GenBank by using ClustalW (www.clustal.org) and manually aligned by using MEGA software version 4.1 (www.megasoftware.net).net
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The CO1 alignment included 3-bp indels in Fumontana and Equitius; these were manually aligned, guided by amino acid translations.
The sequences were manually aligned and the alignments were concatenated.
To generate randomly shuffled control motifs, we manually aligned STAT-related PWMs without gaps by looking up the core regions (TTCCNGGAA).
For evaluation, we used the three MSAs provided with BRaliBase III (5S rRNA, tRNA and U5) and the manually aligned subsets of the MSAs provided by SILVA (SSU and LSU).
A random sample of 35 discordances, involving MeSH descriptors that were manually aligned to VCM, has been reviewed by experts (GK and NG) to assess the reasons for discordance.
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