Sentence examples for manual thresholding of from inspiring English sources

This parameter applies across all frames, removing uncertainties and fluctuations introduced by manual thresholding of individual frames.

Following immunofluorescent detection of insulin and glucagon, Metamorph morphometric software (Universal Imaging) was used to measure the approximate area on each section occupied by either insulin (green channel) or glucagon (red channel) by manual thresholding of pixel intensity.

The PCNA fluorescence channel was next converted to black-and-white using manual thresholding of PCNA intensities.

Nuclei were segmented by converting the DAPI fluorescence channel into black-and-white using manual thresholding of DAPI intensities, and removing areas less than 3 pixels in size.

Finally, the SP-C fluorescence channel was converted into black-and-white using manual thresholding of SP-C intensities, and areas less than 2 pixels in size were ignored.

The Feret's diameter (maximum calliper) was determined using a rolling-ball filter (50 px radius), after correction of the ramp effect and segmentation by manual threshold of the pixel intensity (Schneider et al. 2012).

CT values were collected with a manual threshold of 0.2.

A manual threshold of 0.6000 and an auto baseline were applied in order to obtain the threshold cycle (Ct) for each measurement taken.

Although the software allows automatic controlled thresholding, the preferred method included setting a manual threshold of the CD34-DAB OD for all samples and quantifying endothelial area (EA) using total area measurement option.

These image analysis algorithms do not require manual-thresholding of individual images or time zero image subtraction, due to the use of a 'rolling ball' self-adapting background correction step.

Per day manual threshold selection of the IDOS index resulted in similar amounts of the individual sleep stages (not shown) with a high variance of Cohen's Kappa statistic between recordings.