Sentence examples for manual sequencing of from inspiring English sources
Studies on stage- and gender-specific expression profiles with parasites of various developmental stages have been carried out with different methodological approaches, from manual sequencing of expression sequence tag (EST) to full-length cDNA cloning, microarray hybridization, and random sequencing [15], [16], [17], [18], [19], [20], [21].
Of the phosphopeptides that were identified, manual sequencing of mass spectra unambiguously localized the site of phosphorylation on 549 of the peptides.
Manual sequencing of the corresponding pGEM-T easy constructs for each primer extension was performed using the same primer and employing the Thermo Sequenase™ Cycle Sequencing Kit (USB), following the manufacturer's instructions.
The presence of somatic mutations in the poly(A 10 tract of the transforming growth factor β receptor II (TGFβRII) coding sequence was investigated in MSI-H tumours by PCR followed by direct manual sequencing of the adenosine nucleotide.
Full manual sequencing of the coding region of BRCA1 was conducted in a randomly stratified sample of 91 cases; 47 with, and 44 without, a family history of breast cancer in a first- or second-degree relative.
b To date, 32 and 34 cases included in the T2119C and C3161G analysis, respectively, have been found to have a deleterious mutation in BRCA1 or BRCA2 by protein-truncation testing in specific exons covering about 70% of the coding regions, and by manual sequencing of BRCA1 in a subset.
The CA domains have been identified by visual inspection of the manual sequence alignment of the coronins and motif comparisons with CA domains of WASP family proteins available at CyMoBase (unpublished data, [ 61]).
A thorough manual sequence analysis of the curated list rendered an initial set of 18 IgRs of interest, from which 14 candidates were detected by Northern blot and/or microarray analysis.
A thorough manual sequence analysis of the curated list rendered an initial set of 18 IgRs of interest, from which 14 candidates were detected in strain 2011 by Northern blot and/or microarray analysis.
A detailed manual sequence comparison of eight VgrG proteins listed in their publication showed that four of them contained either repeats or COG4253 at their C-terminal ends.
Genome annotations were completed using DOGMA [ 62] in combination with manual sequence alignments of previously annotated genes from available related species.
