Sentence examples for manual scraping from inspiring English sources
Prior to the process change described below, the workers cleaned the inside of these totes with methylene chloride and manual scraping.
The cells were wounded by manual scraping with a yellow tip.
Protein lysates were collected by the addition of 100 μl of X1 SDS loading dye directly to cells, followed by manual scraping and lysate recovery.
Afterwards, PBS was aspirated and cells were harvested by manual scraping in 40 μl lysis buffer [M-PER (Pierce, Bonn, Germany), Complete Mini, PhosSTOP (Roche, Mannheim, Germany)].
For example, hPSCs can be maintained on Corning® Synthemax™ substrates in mTeSR culture medium [ 10] but a single 6-well plate costs $75 and passaging cells requires manual scraping, which is not amenable to scaled automation.
Four to six hours of this work is very hard but, according to management, the workers are much happier with the current process than they were when working with methylene chloride – a process that involved forceful manual scraping in awkward postures for days at a time.
To identify tumour cell populations for laser microdissection (LM) or manual scrape, malignant cells (cytology specimens) or tissues (paraffin embedded biopsies) were reviewed by a single reference pathologist.
Cells were harvested by manual plate scraping, washed once in cold PBS, and stored at -20°C in RNALater (Ambion; Applied Biosciences, Austin, TX, USA) prior to RNA extraction.
In the case of a small tumor (Patient #20), manual microdissection of the selected area was performed by scraping successive tissue sections under a stereomicroscope.
Although sapphire is a very hard stone, it might be scratched, chipped or shattered by being knocked or scraped during manual activities.
