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This method combines weekly manual sampling and continuous monitoring to provide, qualitative and quantitative information about OM flow.
Of the 12 pairs of manual sampling and automatic sampling compared, ≥ 8 were not significantly different for recovering each of the three groups of indicator organisms (P > 0.05).
In general, good agreement was observed between analyses obtained using the portable FIA system and those obtained from manual sampling and laboratory analysis.
The correlation of the AUC and peak TAC values obtained by manual sampling and from the flow-through detector is shown in Figure 10.
Accurate quantification in PET and SPECT typically requires frequent assessments of blood activity, for example through manual sampling and subsequent measurements in a well counter.
Figure 10 The correlation of the AUC and peak TAC values obtained by manual sampling and from the flow-through detector.
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Concentration measurements after in vitro transcription had a coefficient of variation (CV) of 57% for the manual samples and 1.5% for the automated samples.
The manual sampled and flow-through detector measured blood TACs appeared to be nearly identical as shown in Figure 9 for six different tracers.
Maximum peak values agreed well between manual samples and the detector with a mean difference of −0.4% ± 7.0% (max 12.0%, min −9.9%).
LC/ESI-MS: LC Packings Ultimate HPLC system, with manual sampler and helium degasser.
At set times [ 17, 18], continuous withdrawal was interrupted briefly for collection of manual samples and, after each sample, the arterial line was flushed with heparinised saline.
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