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Manual quantification of nerve fibre morphology is highly labour intensive and requires a trained expert ~10-20 ~10-20age.
However, manual quantification of PMN is operator-dependent and can delay the diagnosis and in fine antibiotic administration.
Manual quantification of 5 cells per embryo of each genotype (wild type, embryos expressing Rab5DN, Rab11DN or both simultaneously in the AS) was performed using ImageJ.
We show here in box-plots that the number of apoptotic neurons increases between stages 13 and 17 in Drosophila embryos (Figure 6), consistently with a previous report of manual quantification of Caspase stained cells [11].
These skin biopsies were analyzed by manual quantification of immunohistochemical double-staining and quantitative RT-PCR.
This screen was based on the manual quantification of response to light touch at the tail of F1 young adult animals.
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To complement the manual quantifications of a restricted number of AS cells over time, we also performed a semi-automated analysis of the entire AS tissue in embryos at the beginning of DC.
Our first approach (manual quantification) requires only visual inspection of treated and control larvae and can thus be carried out using low-power magnification.
However, automated analysis image to assess MVD is a better method than manual quantification because it evaluates larger areas of tumor, is easily reproducible, has higher accuracy and minimizes inter-observer variability [ 28].
The automated quantification of joint effusion from all 25 OA patients gave a mean of 11.7 ± 7.8 ml, whereas 13.5 ± 8.8 ml was obtained by using the manual quantification.
Haematological analysis involves the determination of different blood parameters, which can be done using either the electronic quantification or the manual quantification.
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