Sentence examples for manual pierce from inspiring English sources
The in-gel digestion of proteins was carried out according to the manufacturer's manual (Pierce, Rockford, IL).
Anti-AnxA2 antibodies were first conjugated to sepharose beads according to the manufacturer's manual (Pierce) prior to being used for immunoprecipitation.
The proteins were then transferred electrophoretically to a poly vinylidene difluoride) membrane (Boehringer Mannheim, Penzberg, Germany), and Western blotting was performed as described in the Enhanced Chemiluminescence (ECL) Plus detection system's manual (Pierce, Brockville, Ontario, Canada).
Cerebellum fragments, semi-circular canals, and auditory nerve were removed and whole cochlear lysates were prepared by manual homogenization in lysis buffer containing T-PER Reagent (Pierce), and Protease inhibitor cocktail (Thermo Scientific, Rockford, IL).
Probes were labelled using the Biotin 3′-end DNA Labelling Kit (Pierce) as described in the manual.
The protein concentration was measured with the bicinchoninic acid (BCA) protein assay (Pierce, Perbio Science, Etten-Leur, The Netherlands) as indicated in the user manual, using bovine serum albumin for standard curve.
