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Signal reproducibility is improved with the aid of a sample holder specifically designed for the manual optimization of luminescence signals.
Several differences from previous flow sheets have been found through manual optimization of reflux ratio, number of stripping and rectifying stages, and pressure of the distillation column for typical inlet conditions to the HIx Section III.
That is why the PCR design process is indeed an art, where the manual optimization of the researcher may make the difference and determine the success of a PCR amplification experiment.
Manual optimization of the model yields L = 940 ± 20 nm and d = 9 ± 3 nm.
The percentage identities were obtained from various BLAST programmes without manual optimization of alignments.
Minor manual optimization of alignments, trimming, and management of multi-aligned data was done with BioEdit v7.0.9 [ 42].
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After manual optimization and removal of gaps from the alignments, parsimony, maximum likelihood, and distances were calculated with the 3.63 release of the PHYLIP package [85], using the Jones-Taylor-Thornton matrix and the algorithm of Fitch and Margoliash [86].
Typically, their seed sets are often the outcome of manual optimization procedures for a given read length and number of mismatches.
Therein, the curve of manual optimization is utilized as a reference to compare achieved gains.
Note that the CDF curves of manual optimization are obtained as well by considering joint P0 and Pmax optimization.
Besides, the corresponding UE TP CDFs are depicted in Figure5 along with the curves of manual optimization.
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