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Deglycosylation was conducted with PNGase F as described in the manual instruction.
After electrophoresis, DNA nanotubes were extracted from the gels using Quantum Prep™ Freeze 'N Squeeze DNA Gel Extraction Spin Columns according to the manual instruction.
ALP activity was measured according to the manual instruction.
Total protein concentration was determined by a standard bichromic acid (BCA) assay (manual instruction, Pierce Biotechnology).
Apoptosis was detected by PE Annexin V Apoptosis Detection Kit I according to the manual instruction.
All the samples were tested using two of the tests in the MiniLab® following the manual instruction for each drug.
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All experiments were performed according to the manual instructions provided by the companies.
Factors for depreciation of buildings and equipment and Central Office overhead are added, based on accounting manual instructions.
Reverse transcription was performed with RevertAid RT Kit (Fermentas, Thermo Scientific) according to manual instructions.
During all administrations PedsQL Manual instructions were observed.
The 1st strand cDNA synthesis was performed according to the manual instructions.
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