A 20-μL volume of IC at a predetermined concentration was added to the sample, and DNA was extracted by manual extraction (retrospective study) or automated extraction using the EasyMag extraction system (BioMerieux, Ho Chi Minh City, Vietnam), according to manufacturer's instructions (prospective study).
Overall, cfDNA amounts were higher using manual extraction compared with the automated system, with a mean quantity of cfDNA of 259.43 GE/mL of plasma (range: 61,05 725.33) and 107,35 GE/mL (range: 10,28 327,06), respectively.
By using a manual extraction method to extract corner points, noise error is eliminated and high precision is ensured.
A total of nine matching points from each of the seven calibration boards were extracted via the manual extraction method.
A manual version, the COBAS® TaqMan® Test v2.0 for use with the High Pure System (the HP-TaqMan HCV, v2.0) (Roche Molecular Systems), which instead uses a column-based manual extraction is also available.
RNA extraction from plasma samples were performed using the NucliSENSEasyMAGTM (Biomerieux, Durham, NC) automated nucleic acid extraction system according to the manufacturer's recommendations (NucliSENSEasyMAG user manual, v1.1; BioMe'rieux, Boxtel, Netherlands).
"This is then deposited from the extraction system into a covered skip.
b Dual extraction system.
Manual extraction based on silica columns was found more suitable for viral RNA preparation than an automatic extraction technique.
Based on quantitation cycle values, automated high-throughput extraction of samples proved to be as suitable as manual extraction.
