Sentence examples for manual excision from inspiring English sources

For manual excision, spots were visualized on the gel through silver staining according to Shevchenko et al. [ 47].

For protein visualization, gels were stained with Coomassie G-250 dye (12 h) and destained in 1% (v/v) acetic acid before manual excision of visible protein spots.

Differentially-expressed proteins were then identified by MALDI-TOF-MS and in some cases subsequently analyzed with tandem MS/MS, after manual excision of these protein spots from gels.

In order to identify the proteins by mass spectrometry (MS), the gels were stained with colloidal Coomassie Brilliant Blue according to Kniemeyer et al. (2006) followed by manual excision of the spots.

Manual excision of the protein spots of interest and tryptic digestion were carried out using an Ettan Digester (GE Healthcare, Uppsala, Sweden) and peptide analysis was carried out using a linear ion trap mass spectrometer (LTQ Mass Spectrometer Thermo-Finnigann Ltd, Hemel Hempstead, UK).

In addition, SYPRO Ruby is expensive and stained spots are invisible with the naked eye unless viewed under an ultraviolet or blue light source, making manual spot excision more hazardous and cumbersome [ 97].

Spots of interest were excised from silver-stained gels either by manual or automated excision (ProXCISION; PerkinElmer), reduced, alkylated, and digested overnight with bovine trypsin (Roche), as previously described [23], [24].

Only Kurokawa et al. [13] reported a manual full-thickness excision, but they closed the defect using a stapler, with no description of anti-reflux measures.

Gels to be used for protein identification were run as above and used for both manual and automated excision.

Processing steps included custom protein extraction, SDS-PAGE, manual unbiased band excision, and in-gel digestion with trypsin.

Specifically, the Illumina protocol consists of manual gel band excision, which enhances the occurrence of primer-dimers due to incorrect cutting of the gel or insufficient sample to sequence.