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Non-brain tissues such as skull and dura were stripped with Brain Surface Extractor (BSE) [30], followed by manual editing with ITK-SNAP software [31] to ensure accurate skull removal.
Gels were stained (SyproRuby, BioRad), visualized and analyzed with PDQuest Gel Imaging software (Molecular Imager FX, BioRad), in automatic match mode with manual editing, with images normalized according to overall signal.
However, this position can be corrected by manual editing with high safety through existing error free forward sequence reads.
Lower stringency, however, would likely decrease the reliability of the resultant physical contigs and should be performed by manual editing with the assistance of markers.
Results: A support vector machine (SVM) classifier is trained to reproduce the decisions made during manual editing with an accuracy of 95.0%.
All nucleotide sequences were aligned using the CLUSTAL X program followed by manual editing with various HIV-1 reference subtypes and circulating forms (CRFs) downloaded from the Los Alamos HIV Sequence Database using Bioedit software (available at URL: http://www.mbio.ncsu.edu/bioedit/bioedit.html Accessed 30 January 2013).
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All HIV pol sequences were aligned using Clustal X and manual editing was performed with Bioedit as already described [ 28], removing gaps and cutting to identical sequence lengths.
The results in Figure 1 indicate that, with a window size of 1, the SVM classifier trained on the MANUEL corpus v1.0 can reproduce the manual editing of alignments with an accuracy of 95.0%.
All the sequences were aligned with BVDV reference strains retrieved from GenBank representative of BVDV-1, BVDV-2, and HoBi-like virus using Clustal X; manual editing was performed with Bioedit software version 7.0 (freely available at http://www.mbio.ncsu.edu/bioedit/bioedit.html).html
Exons were predicted with BlastX searches and manual editing based on alignment with cDNA sequences and on the canonical splicing rules (see Figure S2B).
The retrieved entries were clustered and aligned with CAT (Clustering and Alignment Tools) program [39], successively followed by manual edit, alignment with zebrafish genome assemblies, gene prediction with GENSCAN program [40], and BLAST search of zebrafish EST database.
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